Functional Validation

Due to the fact that EVERY protein on the Immunome Protein Array is correctly folded and functional, the results from the platform are highly reproducible and consistent.

Functional Validation – Screening kinases to demonstrate auto-phosphorylation and inhibition with small molecule inhibitors.Func

RET is auto-phosphorylated on the Immunome Protein Array.

Func1

 

  • Proven that proteins are actually functional on the array
  • Kinase auto-phosphorylation and inhibition with small molecule inhibitor
  • Receptor tyrosine kinase potency curves using staurosporine (40µM 0.15nM)
  • Kinase activity correlates with published data

Func2