Highly reproducible within sample
This graph shows the CV variation of the intra-protein replicates run on Immunome slides (red) against Protein Array PX (blue). We can observe that the mean CV% for Immunome was only 3.9% and 37% for the protein array PX.
Immunome is also capable of consistently producing these low CVs across different samples and cases, allowing us to establish the validity of the Immunome array.
Excellent signal to noise ratio
From left to right: the signal ratio for Protein Array PX, as compared to the Immunome array. As you can see, the Immunome signals are much cleaner, and has very low background noise, therefore making it easy to interpretable biomarkers.
*Independent validation data courtesy of KTH, Stockholm.
The graph shows the relationship between the mean RFU and Immunoglobin G (IgG) dilution across different samples. We can clearly see here that they remain consistent across dilution levels and across individual slides. This means that the same RFUs across different slides will be observed if the same dilution levels are used.
The serum antibody detection range limit is in the 10 pg/ml range, although the dynamic range is from 4 to 5 orders of magnitude.
Left to right: Graph showing a near perfect logarithmic relationship between serum dilution and RFU. This means that the relative strengths Right: An image showing protein array spots done at various dilution levels ranging from 1:1000 to 1:128,000