Technology Overview

 

What Is Immunome?

SENGENICS IMMUNOME™ is a patented, novel protein array technology that results in high-throughput and rapid expression of correctly folded proteins by using biotin carboxyl carrier protein (BCCP) as a folding marker and solubility enhancer. Immobilisation of proteins on to any surface is fraught with dangers, particularly if the mode of attachment of the protein to the surface is not tightly controlled. For example, non-specific, physical absorption to a surface through hydrophobic interactions has the potential to be significantly denaturing and to interfere with protein structure and function. At minimum, arrayed proteins will be presented in a heterogeneous manner, leading to a lower specific activity and therefore lower signal-to-noise ratios in assays. By contrast, immobilisation of proteins via an affinity folding marker is a technique employed routinely during the course of affinity purification of proteins prior to functional analysis so there is ample precedent to suggest that, providing the affinity folding marker itself is tolerated, the process of immobilisation on to a surface via the folding marker does not generally affect protein folding or function.    

World’s only correctly folded full-length protein array

The method that is used to make SENGENICS IMMUNOME™ arrays result in high-throughput and rapid expression of correctly folded proteins, with each arrayed protein attached to the surface exclusively via an affinity folding marker, specifically the biotin carboxyl carrier protein (BCCP) domain of the E. coli acetyl CoA carboxylase. The proteins are immobilised onto a solid surface using a unique Biotinylated Carboxyl Carrier Protein (BCCP) folding marker which warrants the correct three-dimensional conformation of the protein and reduces non-specific binding to a minimal level. The protein is biotinylated during natural processing, but only if it is correctly folded. It can be done either in vivo or in vitro, allowing the use of highly specific biotin-streptavidin interaction for surface capture.  To find out about the features of this array in full, please click HERE. Hence, the assay reagents have good access to active sites on the immobilised proteins. These benefits are essential to ensure that data from the protein arrays are reliable and meaningful.

What can this technology do?

This ingenious surface technology of Sengenics’ protein array has been utilised for various medical and therapeutic proteomics applications including autoantibody biomarker profiling, stratification of patients in clinical settings, characterisation of the immunomodulatory properties of antibodies, identification of novel drug targets as well as predictive toxicology. To find out more on some of our possible applications, please take a look at our Applications page.   The standard Sengenics Immunome protein array contains more than 1600 full-length human proteins that represent different classes and various types of proteins such as kinases and transcription factors. The full list of proteins in our standard array is HERE. We also do custom arrays that allows us to correctly express any proteins or isoforms by your request. We also do more than just human proteins – bacterial and viral proteins can also be put into the lysate depending on your application. To find out more, please visit the Custom Array page.  

Applications

Autoantibody Biomarker Discovery Diagnostics and therapeutics biomarker discovery for any condition where an autoimmune response is involved. This includes cancers, autoimmune diseases, autism and most neurodegenerative conditions.

Cancer Immunotherapy Stratifying patients who will go on to have a toxic response to cancer immunotherapy drugs on the basis of their autoantibody signature.

Predicting Vaccine Responsiveness Screening or testing of new vaccines via quantification of interaction between viral proteins and their receptors on human cells, to include measurement of disruption by new vaccines

Infectious Diseases Rapid, high-throughput and sensitive diagnosis of infectious diseases by using custom arrays spotted with viral and bacterial proteins.

Why is Immunome better?

For more details, please visit the Downloads section to get a copy of our standard analysis protocol.