Protein Expression FAQs
KREX™ utilises baculovirus expression system for the production of proteins of interest in insect cells. Although producing these recombinant baculoviruses are technically laborious, it may offers many advantages over other traditional systems: 1) Safety – baculoviruses are nonpathogenic, 2) Ease to scale-up – insect cell suspension cultures allows large-scale protein production in bioreactors, 3) Size – accomodate large genes, 4) High levels of protein expression – variety of efficient gene promoters and 5) Post-translational modification – proper protein folding, glycosylation, phosphorylation, acetylation and acylation.
KREX protein expression system utilises insect cell known as Sf9, a clonal isolate derived from the parental Spodoptera frugiperda cell line IPLB-Sf-21-AE.
4. We have our own proteins of interest. How long does it take to produce these proteins from the DNA sequence provided?
Depending on the size and complexity of the protein, the duration may vary between 3-6 months. Please email us at [email protected] for further discussion.
6. What is the laboratory assay used to validate the infectivity of baculoviruses upon protein production?
Plaque assay is carried out to determine viral titre.
Crude lysates are subjected to SDS-PAGE and the biotinylated proteins were detected via Western blot.