The Fundamental Principle of KREX™

  • KREX proteins cloned in  frame with BCCP,  which acts as a folding  marker and solubility  enhancer​
  • Biotin added to cells and attached to correctly folded proteins by cellular biotin ligases​
  • Misfolded proteins  washed away as they drive co-translational  misfolding of BCCP,  resulting in loss of  function of BCCP​
  • Only correctly folded  proteins are attached  through binding  between BCCP​

KREX™ Technology Overview

Surface chemistry of the KREX™ protein arrays

  • BCCP-tagged, biotinylated KREX proteins are immobilised onto customised  streptavidin-coated hydrogel​
  • KREX proteins retain folded structure and function in an aqueous environment​
  • PROTEINS behave as if they are in free solution​
  • Simultaneous purification and immobilisation – “Surface Capture”


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