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    Autoantibody Biomarker Discovery

    H-Pylori – Parkinson’s Disease Study

  • Study Design:

    • Investigate the association of H-pylori in PD patients: 30 H. pylori-seropositive PD samples were designated as case and 30 age- and gender- matched H. pylori-seronegative PD samples were used as controls
  • Results:

    • Study identified 13 significant autoantibodies based on ranking using a penetrance fold change method.
    • Among elevated autoantibodies in H. pylori-seropositive PD, NFIA, PDGFB and eIFA3 are essential proteins involved in neurological function.
  • Published study:

    Augmentation of Autoantibodies by Helicobacter pylori in Parkinson’s Disease Patients May Be Linked to Greater Severity

    Fold changes between H. pylori-seropositive PD samples (Case) and H. pylori-seronegative (Control) groups for autoantibodies showing higher activity in the case group.

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    Drug Companion Diagnostics

    Checkpoint Inhibitor Melanoma Trial

  • Study Design:

    • Metastatic melanoma, BRAF mutant patients, treated with either:
      1. Ipilimumab (anti-CTLA-4)
      2. Pembrolizumab (anti-PD-1)
      3. Nivolumab (anti-PD-1)
      4. Ipil & Nivo
  • Results:

    • ~58% response rate in nivo plus ipil patients, but high grade (3 or 4) irAEs occur in ~55%
    • The combination of nivolumab and ipilimumab resulted in numerically longer progression-free survival and a higher rate of response than did nivolumab alone in the overall study population
    • The incidence of adverse events in this study was, in general, lowest in the nivolumab group and highest in the combination group
  • Published study:

    Autoantibodies May Predict Immune-Related Toxicity: Results from a Phase I Study of Intralesional Bacillus Calmette-Guérin followed by Ipilimumab in Patients with Advanced Metastatic Melanoma

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    Protein Interaction Assays

    DNA-Protein Interaction Study

  • Study Design:

    • p53 microarray printed onto a neutravidin-derivatised dextran hydrogel surface probed with Cy3-labelled GADD45 duplex oligo
  • Results:

    • Quantitative parallel thermodynamic analysis revealed previously unknown functional effects of mutation on p53 protein-DNA interaction
    • Differential DNA binding activity of p53 mutant proteins
    • Quantitative analysis unravels mechanistic differences between ‘loss of function’ mutations
  • Published study:

    Functional protein microarrays for parallel characterisation of p53 mutants

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