Technical Performance of our Immunome Protein Array

Technical Performance

Exceptional consistency

Protein arrays made with the KREX technology exhibit exceptional consistency as measured by the coefficient of variation percentage (CV%).

This graph shows the CV% of the intra-protein replicates run on the KREX-based Immunome protein array (dark blue) against a non-KREX protein array PX (light blue). A mean CV% for Immunome of 3.9% and 37% for protein array PX were observed.

Excellent signal-to-noise ratio

The signal-to-noise ratio on KREX-based protein arrays is excellent. Signals are much cleaner with very low background noise and easily interpretable hits.

Highly reproducible

KREX-based protein arrays use multiple positive and negative controls to measure reproducibility within, and across, studies and batches.

The graph below shows an almost perfect Pearson correlation of above 0.97 when comparing autoantibody levels across 6,524 protein data points in two different batches.

Picomolar sensitivity

Autoantibodies on KREX-based protein arrays can be detected at very high sensitivity, only 1 – 10 µl of serum is required. The detection limit is in the 10 pg/ml range, with a dynamic range of 4 to 5 orders of magnitude.