Homodimers
co-immobilised
Homodimers
co-immobilised

Proteins naturally
assemble
Proteins naturally
assemble

Transmembrane proteins expressed
Transmembrane proteins expressed

Functionally
verified proteins
Functionally
verified proteins

As all proteins on the Immunome protein array are correctly folded, they naturally conform into their correct native state upon immobilisation on the array. Hence they are functional, physiologically active and are able to carry out their specific roles and functions. For example:

  • Kinases that are required to be present as homodimers are catalytically active, form homodimers, and autophosporylate in the presence of ATP.

  • DNA binding proteins are able to bind fluorescently labelled DNA oligos.

Homodimers co-immobilised on the array

On-chip phosphorylation assays demonstrate functionality of arrayed kinases.This example demonstrates that human kinases on the array are present as homodimers (as evidenced by autophosphorylation in the presence of ATP, which is required for catalytic activity)

Homodimers-co-immobilised-on-the-array

Proteins naturally assemble

Many proteins exist in multimeric complexes, and will only function when individual monomers form a complete unit. Homodimers and tetramer components interact with each other on the array, and form functional complexes in which both conformational and linear epitopes are present.On-array trimers and tetramers form naturally on the Immunome protein array.This allows quantification of Protein-protein interactions and disruptions

Transmembrane proteins expressed

Full length membrane proteins can be expressed and immobilised. Differential detergent extractions or sonication are then used to partially disrupt the membranes, allowing the BCCP-tagged protein to be immobilised onto a streptavidin surface. The proteins are left embedded in the membrane, in their native conformation

Functionally verified proteins

Kinase inhibition assays

Iressa Study

Iressa is a small molecule lung cancer drug that inhibits EGFR tyrosine kinase activity. The Immunome protein array was used to evaluate the specificity of Iressa-mediated kinase inhibition. The results below clearly show that Iressa also inhibits the activity of many other kinases. Hence, this assay can be used to predict toxicology due to off-target events.

DNA binding assays

P53 study

A custom array consisting of various mutated versions of the p53 oncoprotein was probed with a cy-3 labelled GADD45 duplex oligo. Differential DNA binding activity can clearly be observed, demonstrating once again that proteins on the Immunome protein array platform are functionally active.

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